奶牛乳源大肠杆菌耐药性、毒素基因鉴定及PFGE分型研究

doi:10.11843/j.issn.0366-6964.2014.03.017

Abstract

Abstract:

The aim of this study was to investigate bacterial antibiotic resistance，virulence genes and PFGE patterns of 28 Escherichia coli strains isolated from 197 samples (including 195 milk samples，and 2 cloth towel water samples that were used for cleaning cow teats) from a Northern Chinese Holstein farm.Antimicrobial susceptibility testing results of the 28 E.coli strains to 35 antibiotics showed that gentamicin and spectinomycin could be used as common clinical veterinary antibiotics in this farm.However，special attention should be paid to the emergence of broad-spectrum medemycin resistance in this farm.Since E.coli strains isolated from cloth towel water samples also had strong resistance to antibiotics，the farm manager should take actions to prevent cloths towel transferring mastitis.For the 28 E.coli strains，the detectable rate of virulence gene Ecs3703 was 100%，which implied that they are Enterohemorrhagic E.coli (EHEC) rather than Enterotoxigenic E.coli (ETEC).And those E.coli strains which carry virulence genes Irp2 and/or CNF2 might be the main pathogen of dairy cattle mastitis.

CLC Number:

S852.612

WANG Xiao，WANG Xin，HAO Dan，TANG Xiao-shuang，ZHOU Chuan-li，WANG Ya-chun，ZHANG Sheng-li，ZHANG Yuan，YU Ying. Study on Antibiotic Resistance，Virulence Toxin Genes and PFGE Patterns of Escherichia coli Strains Isolated from Dairy Cattle Milk[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 463-470.

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References

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Study on Antibiotic Resistance，Virulence Toxin Genes and PFGE Patterns of Escherichia coli Strains Isolated from Dairy Cattle Milk

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